Plasmid

Part:BBa_K5371205:Design

Designed by: Haoyang Yu   Group: iGEM24_iZJU-China   (2024-09-29)


pRS42H-TSC10-EGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 836
    Illegal EcoRI site found at 2984
    Illegal XbaI site found at 8358
    Illegal PstI site found at 926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 836
    Illegal EcoRI site found at 2984
    Illegal PstI site found at 926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 836
    Illegal EcoRI site found at 2984
    Illegal BamHI site found at 3938
    Illegal XhoI site found at 2671
    Illegal XhoI site found at 4664
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 836
    Illegal EcoRI site found at 2984
    Illegal XbaI site found at 8358
    Illegal PstI site found at 926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 836
    Illegal EcoRI site found at 2984
    Illegal XbaI site found at 8358
    Illegal PstI site found at 926
    Illegal NgoMIV site found at 2415
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2572
    Illegal BsaI.rc site found at 6528
    Illegal SapI site found at 5445


Design Notes

Stop codon of TSC10 was removed for continuous and complete expression of TSC10-P2A-EGFP to prevent a premature stop in this case.


Source

pRS42H-TSC10-EGFP was constructed via Gibson Assembly, with segments mainly amplified from Saccharomyces cerevisiae.

References